Anesthetics are typically used in preclinical functional magnetic resonance imaging (fMRI) experiments to prevent animal movement and stress, but anesthetics can significantly affect the brain function. Therefore, several awake fMRI protocols have been developed, usually implementing house-made coils and holders. In present study, we developed a 3D printable restrainment kit that is compatible with standard Bruker rat coils and holders. Subsequently, resting state functional connectivity (RSFC) data was obtained under awake, lightly sedated, and anesthetized conditions. Our results show that even subanesthetic dose of isoflurane modulates FC significantly.