Relative cerebral blood volume (rCBV) obtained from dynamic susceptibility contrast (DSC) MRI is adversely impacted by contrast agent leakage in brain tumors. Using a digital reference object, we previously demonstrated that multi-echo DSC-MRI protocols provide advantages in terms of contrast agent dosing, pulse sequence flexibility, and rCBV accuracy. The purpose of this study is to assess the in-vivo performance of multi-echo acquisitions in patients with brain tumors. Multi-echo rCBV obtained without a preload is compared to the standard single-echo rCBV obtained with preload. In addition, two contrast agent doses and two flip angles are compared for the multi-echo acquisition.