Myocardium ischemia irritates a series of metabolic degradations, including intracellular acidosis and cell damage. Amide proton transfer (APT) effect is associated with microenvironment acidosis and amide protons inside of cytoplasm. In this study, APT imaging was performed on MI patients at 3 Tesla. Results show significant APT effect reduction from the remote (4.29±2.00%), adjacent (2.74±1.18%) to the infarct (1.43±0.80%) regions (P<0.05), implying progressively metabolic alterations from surrounding tissue to the infarct core. The method provides a novel way for noninvasive assessment of myocardium metabolic degradation postinfarction, promising to facilitate therapeutic decision and treatment evaluation.