We investigated whether Deuterium Metabolic Imaging (DMI) with 2H-labeled glucose can be used to monitor treatment response in a pre-clinical murine lymphoma model (EL4). Localized 2H spectra were acquired from tumors, with a time resolution of ~1min, following a bolus injection of labeled glucose. These showed inhibition of lactate labeling following treatment of the tumors with etoposide. 2D chemical shift images (2D CSI) were acquired from the tumors, but imaging treatment response may need faster image acquisition. Studies with a respiratory chain inhibitor in vitro suggested that labeling of water in EL4 tumor cells may be due, at least in part, to TCA cycle activity.