Glaucoma is the second leading cause of blindness worldwide. To date, the cause of the disease is still unknown, yet a number of candidate genes for glaucoma have been recently identified. In this study, we evaluated the effects of some of these genetic risk factors on the visual pathway using diffusion tensor MRI in experimental animal models. We found that LTBP2 mutants in cats and DBA/2J mice had decreased fractional anisotropy and increased radial diffusivity in the visual pathway. DBA/2J mice also exhibited reduced axial diffusivity, whereas TBK1-transgenic mice did not show detectable DTI changes along the visual pathway.