Laminar-specific functional magnetic resonance imaging (fMRI) opens new possibility for studying the neuronal circuitry and functional connectivity of cortex. FLASH based line-scanning method was proposed with high temporal and spatial resolution to better characterize the fMRI onset time across the cortical layers by combining 2 saturation RF pulses. However, the imperfect RF saturation performance led to poor boundary definition of the ROI from the cortex. In this work, we propose an α-180˚ SE based line-scanning (SELINE) method, solving this problem. We will improve this method to better understand the distinct laminar-specific neuronal circuitry and functional connectivity.