In this study we explored the feasibility of using an MR-compatible ergometer mimicking stair climbing to study differences in T2* after exercise between and within different thigh muscles. Four healthy subjects performed a 10-minute stepping exercise inside a 3T. Participants exercised at either a high rate of perceived exertion (RPE) or a low RPE. Clear differences between low and high RPE and different muscles, but not within muscles, were found. This shows that it is possible to use stair-climbing using an MR-compatible ergometer to study differences between and within muscles in response to exercise.