We focused on aquaporin 4 in skeletal muscle and attempted to visualize its function using time-dependent diffusion magnetic resonance imaging (MRI). In addition, the validity of the muscle cell type characteristics was evaluated by immunostaining. The diffusion time was adjusted with the PG-STE method using a 9.4-T MRI scanner. Diffusivity associated with a difference in the diffusion time was found to differ depending on the skeletal muscle type. Thus, it was possible to visualize the water molecule exchange rate of skeletal muscle cell membranes.