Synthetic MRI enables absolute quantification of T1, T2 and (proton density) PD value. The aim of the study was to primarily evaluate the feasibility of synthetic MRI in differential diagnosis of (prostate cancer) PCa. We analyzed 18 PCa lesions in 14 PCa patients, 26 SH (stromal hyperplasia), 25 GH (glandular hyperplasia) nodules and 21 prostatitis areas in 22 non-PCa patients who underwent multi-parameter MRI before needle biopsy. T1WI, T2WI, DWI and MAGiC (magnetic resonance image compilation) sequences were acquired respectively. Our results showed the T1 and T2 value of PCa lesion was significantly lower than GH nodule and prostatitis area. The PD value of PCa lesion was significantly lower than GH nodule. We concluded that synthetic MRI was helpful for differential diagnosis of PCa.