The noninvasive assessment of renal oxygenation is of great clinical interest. Some recent studies used a single shot asymmetric spin echo (ASE) EPI approach to measure oxygen extraction fraction (OEF) in the brain. Compared to the brain, the abdomen suffers much larger field inhomogeneity and respiration induced motion artifacts. The ASE EPI sequence was optimized by shifting the readout position rather than the 180° RF pulse to reduce TE and hence susceptibility artifacts for better OEF measurement in the kidney. OEF results were in good agreement with the normal range of about 30%, as reported in the previous literature.