Hyperpolarized [1-13C]pyruvate was used to evaluate metabolic and perfusion changes in myocardial infarction in the critical acute reperfusion phase. Regional changes was observed by mapping of metabolic ratios of lactate, alanine and bicarbonate. Metabolic exchange rates from hyperpolarized fumarate was calculated using Michael-Menten kinetics. Hyperpolarized measures were compared with standard perfusion MRI and tissue staining evaluating infarction and salvage. The results of this study, draws use of hyperpolarized [1-13C]pyruvate closer to evaluation of myocardial infarction in a human study.