There is a growing preclinical demand for methods to characterize atherosclerotic plaque in vivo. Recently proposed DANTE for dark-blood preparation successfully in vessel wall imaging. However, previous studies were performed at 3T and used for vessel wall imaging of human. It remains unclear that if DANTE module could be used for apoE-/- mouse at 7T. Here we demonstrated a sequence using DANTE dark-blood preparation combined with FLASH readout (DANTE-FLASH) for three-dimensional (3D) vessel wall imaging at 7 Tesla (T).