Zebrafish has been a widely used model to study cardiovascular development and diseases. Among the multiple advantages, zebrafish has the remarkable ability to regenerate lost or damaged organs including the heart. In vivo visualization of the cardiovascular system is particularly easy during early stages of development due to zebrafish transparency, which is lost in the adult stages. Therefore, it is of great interest to refine and adapt non-invasive imaging methods that allow a functional assessment of the zebrafish adult heart. The application of high-field functional MRI of the beating zebrafish heart will be presented and discussed.