Here we report extracellular pH (pHe) mapping with BIRDS using TmDOTP5- in normal and VX2 tumors in rabbit liver tissue. Transarterial chemoembolization (TACE) was performed and the rabbits were scanned without TACE, or at 1 day and 2 weeks post TACE. The pHe maps show lower pHe in tumor and tumor edge compared to normal liver. Tumor acidity prior to TACE remain at 1 day post TACE, but it is almost normalized at 2 weeks post TACE. The ability to measure pHe in a translational model and compare it with “normal” tissue improves tumor detection and monitoring of tumor treatment.