The routine clinical technique for prenatal diagnosis of cleft palate (CP) is ultrasound (US). However, the technique has difficulties on definitive diagnosis of fetal CP especially cleft posterior palate because of its technical limitations. Previous studies have demonstrated that magnetic resonance (MR) imaging can be a useful adjunct to the diagnosis of fetal CP and provides better diagnosis performance than US. However, these studies are mainly based on 2D MR imaging which has limited resolution along slice selective direction and cannot freely visualize the fetal palate from any orientation. To address this issue, we sought to use a 3D super-resolution reconstruction method to reconstruct 3D isotropic volumetric images from 2D images stacks and evaluate its feasibility of CP diagnosis.