Cancer induced cachexia is a syndrome characterized by tissue wasting and weight loss. Cachexia occurs with the highest frequency and severity in pancreatic ductal adenocarcinoma (PDAC). To further understand this syndrome, we used 1H MRS to analyze pancreas metabolites in mice with and without cachexia-inducing PDAC xenografts. We detected significant weight loss in cachectic mice. 1H MR spectra identified significant differences in amino acids, BCAA, alanine, pyruvate, phosphocholine, niacinamide and NAD in cachectic mice that provide new insights into the morbidity and mortality associated with the syndrome that may lead to novel strategies to arrest this syndrome.