Major depressive disorder (MDD) is a common mental disorder characterized by cognitive and affective deficits. Prior works indicated that anterior cingulate cortex (ACC) is related to high-level cognitive and emotion process, which is also thought to be pivotal to depression. Here, we examined the resting FC of ACC sub-regions in fist-episode MDD patients. The current results revealed reduced ACC sub-regional FC with IPL and SPL while increased FC was found in dmPFC. Additionally, FC with IPL also negatively correlated with symptom severity (HDRS), indicating that depression may disrupt the normal interactions within the DMN. These findings on alteration of ACC sub-regional FC may contribute to the comprehension in pathophysiology of MDD.