Gliomas of different histopathological grades have diametrically different prognosis and need biopsy-verification for diagnosis. Amide Proton Transfer weighted imaging (APTw), a non-invasive CEST MRI technique, was utilised for tumor differentiation of low grade and high grade brain gliomas. Mean lesional APTw-effect was quantified in regions with highest signal in 20 individuals. The information proved to be useful as the findings demonstrated that APTw increased the diagnostic accuracy in the clinical setting which may reduce treatment delay and improve diagnostics. APTw-imaging therefore shows promise as a clinical tool.