Accurate glioma grading is crucial for therapeutic strategy and prognosis. T1ρ MRI could probe the interactions between motion-restricted water and macromolecules in tissues. Our study aimed to determine the diagnostic efficiency of T1ρ MRI in glioma grading. Significant differences were found by rT1ρ between low- and high-grade, WHO grade Ⅱ and Ⅲ, WHO grade Ⅱ and Ⅳ gliomas when we placed the ROIs in the solid portion and the peritumoral portion respectively. rT1ρ demonstrated a high diagnostic performance in the solid portion and a moderate diagnostic performance in the peritumoral portion in grading. T1ρ MRI has potential to be a noninvasive quantitative method for preoperatively grading gliomas.