This study aimed to explore the value of MR textural analysis in assessing consistency of pituitary macroadenoma (PMA) based on contrast enhanced (CE) 3D-SPACE images. Fifty-three patients with PMAs that underwent CE 3D-SPACE scanning and endoscopic trans-sphenoidal surgery were included. First-order energy and second-order correlation negatively correlated with hard PMAs, while first-order entropy and second-order variance, sum variance, and sum entropy positively correlated with stiffness (P<0.05). Diagnostic accuracy of combined negative features could achieve an AUC of 0.819, and an AUC of 0.836 of positive features (P<0.001). MRTA using CE 3D-SPACE images is helpful for assessing PMA consistency preoperatively and noninvasively.