Preoperative localization of the normal pituitary gland is important in patients with pituitary adenoma. Our aim was to evaluate the possible role of high-temporal and spatial resolution dynamic contrast enhanced MR imaging (DCE-MRI) and quantitative pharmacokinetic analysis in differentiation of the normal pituitary gland from pituitary adenoma. The normal pituitary gland showed significantly higher IAUC, Ktrans, kep and ve than pituitary adenoma. The ROC curve analysis showed significance for IAUC, Ktrans, kep and ve (AUC = 0.958, 0.882, 0.781 and 0.851, respectively). These quantitative parameters may be useful for differentiation of the normal pituitary gland from pituitary adenoma.