The purpose of this study is to explore the alterations of IVIM measurement among multiple b-value sets with different low b-value and high b-value numbers using EPDWI. EPDWI with 41 different b-values was performed. For simulating the situation in clinic, five sets of 16 b-value numbers were chose from the 41 b values. DWI data of 1000 pixels for each WM, GM and CSF were randomly chose to evaluate the error of IVIM parameters. Our study demonstrated the variation of DWI signal with multi-b-value, affect the evaluation of Df and f but does not affect Ds in pixel-wise IVIM analysis.