The purpose of this study was to evaluate the capability of optimized and faster ultra-high b-value DWI in separating and identifying intrathalamic substructures compared to a previously described protocol. 7 subjects underwent MR-imaging, including T1 MPRAGE and two DWI sequences at 0 and 5000 s/mm2. DWI was performed with 64 directions and 5 averages (17:56 min) and with 6 directions and 25 averages (8:23 min). Intrathalamic substructures were semi-automatically delineate 4mm above AC/PC line. Accordance between the original sequence and the new speeded-up measurement was high. Acquisition time was reduced by more than 50 % with comparable results.