This study examines the impact of dietary fatty acid intake on functional connectivity of the maturing brain. This study was performed with 88 rats. In vivo as well as ex vivo neurological data was collected by means of MR imaging and postmortem gas chromatography. The graphs were compared by using network-based statistics and showed non-significant trends between the controls and the fatty-acids deficient group. Network metrics were also computed and showed non-significant trends between the controls and the fatty acids deficient group.