Longitudinal cerebral blood flow (CBF) images were acquired in ten college students after 2-month meditation practice. The CBF difference before and after meditation practice and the association between CBF change and meditation practice time were analyzed on a voxel-by-voxel basis using SPM8. We found significant perfusion increase and decrease in the occipital and thalamus regions respectively. However, the CBF increase and decrease in the corresponding regions exhibited negative and positive association with practice time respectively. Regional analysis confirmed that the direction of CBF change may switch after some amount of meditation practice time, indicating a complex neural pathway of meditation.