The sustained effect of meditation on brain functional connectivity (FC) was evaluated longitudinally in ten college students. FC maps before and after meditation practice were compared and the changes of FC were correlated to practice time. We observed a significantly increased FC between the default mode network (DMN) and the dorsal attention network (DAN), and within DAN. The increased FC between DMN and DAN and within DAN was associated with increased practice time. The findings support fast shifting of attention after practicing meditation for two months and lay a foundation for its clinical transformation if confirmed in a larger study.