Breast cancer is one most common cancer in women worldwide. Human epidermal growth factor receptor-2（HER-2）is an important biomarker whose expression status closely relates to the disease outcomes in patients with breast cancer. This study retrospectively explored the difference of MRI time-signal intensity curve types between patients with HER-2 overexpression and patients without HER-2 overexpression. The results indicated the type of washout time-signal intensity curve(TIC) and Non-tumor strengthening is more common in HER-2 amplification or overexpression. The MRI TIC can assess Her-2 status of amplification or overexpression non-invasively before surgery ,and this can help to guide clinician treatment decision and prognosis evaluation.