This work aims to investigate the initializations of R2* and field maps in R2*-IDRAL for developing a robust quantitative susceptibility mapping (QSM) in the spine. A 3D multi-echo GRE sequence was implemented to acquire out-phase and in-phase (IP) echoes in 10 subjects. The R2* and background field maps estimated by fitting the magnitude and phase of IP echoes were used to initialize R2*-IDEAL to obtain final R2*, field, water, and fat maps. The final field map was further processed to generate QSM. The results demonstrated that IP initializations of R2* and field in R2*-IDEAL provide robust QSM of the spine.