Quantitative Susceptibility Mapping (QSM) has recently been used for assessing the cerebral oxygen metabolism. However, a systematic investigation on the most suitable imaging parameters and reconstruction algorithms for determining the venous susceptibility values is missing. Therefore, we investigated both, the impact of flow compensation and accelerated acquisition as well as different reconstruction methods on measured venous susceptibility. Our results suggest that the choice of reconstruction technique can significantly influence the venous susceptibility values while the investigated imaging parameters did not considerably affect its accuracy. Thus, the applied QSM reconstruction technique has to be considered carefully when quantifying the venous oxygenation.