In this study, the influence of the cardiac cycle on the amount of label produced by velocity-selective (VSASL) and acceleration-selective arterial spin labeling (AccASL) was investigated. A sequence combining pCASL and VSASL(AccASL) was developed to isolate the arterial blood pool. Results showed significant arterial signal fluctuations in the amount of label produced by VSASL, AccASL and pCASL over the cardiac cycle. Hence, in order to become independent of the cardiac cycle, sufficient averages need to be taken when applying these techniques. Alternatively, these findings could be highly interesting for the purpose of quantifying pulsatility higher up in the vascular tree.