Velocity-selective arterial spin labeling (VSASL) employing Fourier-transform based velocity-selective pulse trains is an emerging method for quantifying cerebral blood flow (CBF) with high sensitivity to perfusion signal. Its utility was assessed for glioma patients at 3T by a comparison between pseudo-continuous ASL with DSC-PWI. We demonstrated the existence of various and prolonged arterial transit time (ATT) in high-grade gliomas. Detecting by the dependence of the CBF based on Tmax, lesser sensitivity to ATT in VSASL was reported. VSASL showed great promise for accurate quantification of CBF and could potentially improve the diagnostic performance of ASL in preoperative grading of gliomas.