To obtain low-frequency anisotropic conductivity distribution of biological tissues recently Diffusion Tensor Magnetic Resonance Electrical Impedance Tomography (DT-MREIT), which is combination of the DTI and MREIT techniques, is proposed. There are two in vivo applications of DT-MREIT in the literature where two linearly independent current injection patterns are used. Decreasing the number of current injection patterns to one improves the feasibility of DT-MREIT in clinical applications. In this study, DT-MREIT using a single current injection pattern is experimentally realized. The obtained results approve the validity of the proposed single current DT-MREIT method.