We simultaneously quantified acidity and hypoxia of human gliomas with IDH, 1p/19q, and EGFR genotypes using CEST-SAGE-EPI. Results suggest IDH mutant gliomas are significantly less acidic and hypoxic than IDH wild-type tumors. Within IDH mutants, 1p/19q codeletion is associated with lower tumor acidity, while IDH wild-type, EGFR amplified tumors were more hypoxic. Both MRI-derived acidity and hypoxia were correlated with patient survival, suggesting metabolic characteristics may be prognostic. CEST-SAGE-EPI may be useful for exploring metabolic changes that result from particular genetic alterations or useful as a biomarker for accelerating drug development in human brain tumors.