Accurate identification of isocitrate dehydrogenase (IDH) mutant high-grade glioma is clinically important. We investigated the combined utility of diffusion (DTI) and perfusion (DSC-PWI) MR imaging in distinguishing IDH mutant from IDH wild-type high-grade gliomas. Treatment naïve patients (n=30) with IDH-mutant (n=14) and IDH-wild-type (n=16) high-grade gliomas were recruited. A classification model comprising of mean diffusivity, coefficient of planar anisotropy and maximum relative cerebral blood volume differentiated two genotypes of gliomas with an accuracy of 85%, a sensitivity of 87.2%, and a specificity of 81.5%. Combined analysis of DTI and DSC-PWI may be helpful in distinguishing IDH profiles of high-grade gliomas.