19F-MRI of inhaled perfluoropropane can be used to assess regional pulmonary ventilation. We conducted a prospective multi-centre reproducibility study in 40 healthy volunteers. Same-day static breath-hold 19F-MR images with 1 cm isotropic resolution were acquired on four occasions for each volunteer following inhalation of a perfluoropropane/oxygen gas mixture. Percentage ventilated lung volume (%VV) was calculated for all volunteers, reflecting the inhalation protocol, imaging protocol, and image registration and segmentation process applied. Volunteer %VV was determined to within ±1.7% (95% CI). Gas inhalations were well tolerated by all volunteers with no adverse events.