This study was to prospectively evaluate APT imaging for the parotid glands and lesions. 32 patients, confirmed cancer in parotid glands, underwent 3D TSE APTw imaging. Scores for integrity and for hyperintensity artifacts of both tumor lesions and normal parotid glands were evaluated. Tumor lesions had better integrity score than normal parotid glands. Scores for hyperintensity artifacts in APTw images showed no significant difference between tumor lesions and normal parotid glands. Most APTw images of parotid glands lesions were scored with good integrity and had acceptable image quality, while challenges still exist in some cases.