The vascular effects of e-cigarette use were investigated in young adults (19-35 years). Blood draws and 3T-MRI data were collected from seven e-cigarette users, seven smokers, thirty nonsmokers, the latter replicating the measurements after one nicotine-free e-cigarette vaping session. MRI-protocol measured peripheral vascular reactivity in response to cuff-induced ischemia, quantifying femoral artery luminal flow mediated dilation (FMDL), blood flow velocity, venous saturation (SvO2). FMDL decreased by 33% acutely after vaping, consistent with 20% NOx reduction and elevated inflammation (C-reactive protein increased by 95%). Reactive hyperemia was blunted as a chronic effect of both smoking and vaping, paired with anomalous biomarkers.