Atherosclerosis is the predominant risk factor of ischemic stroke. Age and location are intrinsic factors in the development of carotid artery plaque. Therefore, the relationship between hemodynamic changes and location/age in healthy subjects can help understand how the hemodynamic states influence carotid incidents. Our study found velocity/WSS/PG decreased with age. And proximal ICA, the most common point arising atherosclerotic plaque, displayed the lowest velocity/WSS/PG. It may imply this state of hemodynamics are more likely to cause atherosclerotic plaques. The multi-parameter analysis of 4D flow MRI may offer suggestions for choosing age and location matched control cohorts in future study.