Radiomics provides a tool for comprehensive quantification and visualization of intra-tumor heterogeneity at the radiological level. Several radiomics studies have been reported in prediction of the survival and treatment response of glioma. And most researches had focused on binary classification of the Low-grade glioma (grade II) and high-grade glioma (grade III and grade IV). However, the influence of different MRI scan plane on the radiomic features of glioma has not been investigated. The purpose of this retrospective study was to demonstrate the feasibility of radiomics methods to determine the three subtypes (grade II, III, and IV) of glioma based on multi-sequences and different scan plane of magnetic resonance imaging (MRI).