Heart failure with preserved ejection fraction is accompanied by diastolic dysfunction. Diastolic dysfunction is associated with metabolic dysregulations, particularly obesity. Physical Activity might reduce this process improving diastolic function and left ventricular (LV) morphology. In this cross-sectional Netherlands Epidemiology of Obesity study, individuals underwent cardiovascular MRI to asses diastolic function and LV Morphology. Physical Activity was determined via the SQUASH questionnaire. Multivariable linear regression was performed adjusting for metabolic covariates. Physical Activity was moderately associated to diastolic function and strongly to LV Morphology. Conclusively, associations of diastolic function and LV morphology with Physical Activity can be determined accurately by MRI.