This study evaluated if multiparametric magnetic resonance imaging (MRI) could identify changes in warm ischemia-reperfusion injury (WIRI) after liposomal prostaglandin E1 (Lipo-PGE1) intervention in rabbit livers. We measured blood oxygen level–dependent, diffusion tensor, and intravoxel incoherent motion imaging on functional hepatic parameters in rabbits exposed to 1) warm ischemia at different times, 2) Lipo-PGE1 interventions, and 3) sham operations. The parameters were sensitive to perfusion and oxygenation changes during WIRI. Multiparametric MRI successfully identified changes after Lipo-PGE1 intervention. These results offer new insights to quantitatively evaluate the effect of drugs on hepatic WIRI after liver transplantation and hepatectomy.