Mutations in the X-linked dystrophin gene disrupts protein expression resulting in Duchenne muscular dystrophy (DMD), a neuromuscular disorder characterized by body-wide muscle cell degeneration. The mdx mouse model is one of the most commonly used animal models for DMD. Recombinant adeno-associated viral (rAAV) vector-mediated gene transfer represents a promising approach for DMD. Magnetic resonance has emerged as a noninvasive method in monitoring disease progression and treatment response for muscular dystrophy. The aim of this study was to elucidate the functional impact of micro-dystrophin on skeletal muscles using magnetic resonance imaging and spectroscopy.