Magnetic resonance spectroscopy (MRS) offers a wide range of methods to study in vivo metabolism. As a result of this diversity, data acquisition and analysis are far from standardized, and researchers frequently develop customized and therefore highly heterogeneous pipelines, often interfacing with commercial or closed-source external fitting software. Here, we present “Osprey”, an all-in-one software package that incorporates all steps of state-of-the-art pre-processing, linear-combination modeling, tissue correction, quantification, and visualization of MRS data from the human brain. Osprey provides a modular, fully open-source framework, allowing the community to rapidly incorporate future methodological developments, accelerate their adaptation, and foster standardization.
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