Oxidative stress is implicated in various diseases such as inflammation, neurodegenerative disorders (Alzheimer’s disease, Parkinson’s disease), atherosclerosis, diabetic and cancer. Excess reactive oxygen species (ROS) are produced during altered cellular metabolism in various diseases. Among ROSs, hydroxyl radicals (•OH) is one of the most reactive molecules in biological systems. Therefore, it is considered that monitoring of •OH is could be useful technologies for evaluation of redox mechanism and oxidative diseases. In this study, we developed the hydroxyl radical imaging technique using combining DNP-MRI and DMPO.