We implemented a diffusion-weighted semi-LASER single voxel spectroscopy sequence with no water suppression to measure mean diffusivity and fractional anisotropy within two volumes-of-interest in the corpus callosum. Without water suppression, we were able to decrease acquisition time and use the water signal for phase, frequency, and eddy current corrections. We measured the signal decay of N-acetylaspartate (tNAA), creatine (tCr), choline (tCho), and water to derive diffusion outcomes and compare between the two volumes-of-interest. Our implementation was done in feasible time for in vivo measurements. Further development can lead to more specific assessments of brain microstructure and pathophysiology.