2D L-COSY has previously been used to demonstrate neurochemical differences between PTSD and healthy cohorts but requires a 19 minute acquisition. Single Voxel Spectroscopy (SVS), acquired in 3 minutes, was used to collect data from 3 brain regions in healthy controls and patients with Post-traumatic Stress Disorder. Neurochemical differences were seen in all the ACC, Thalamus and PCG. Data, analysed using LC Model, showed elevated levels of NAA, Lactate, GABA and Glutathione as well as glutamatergic dysfunction. Thus, SVS can be used to identify PTSD in a shorter time frame than 2D L-COSY.