In this study we show the feasibility of simultaneous
measure of oxygen consumption and metabolic data in living 3D cell culture in
standard 5mm NMR tube. Using high cell density of 20 million fibroblasts we
demonstrate high viability and reproducibility of the measurements over 12
hours. We describe the effect of the flow rate on the metabolic activity.
Importantly, we show the required sensitivity to detect substrate degradation
rates of major mitochondrial fuel pathways and ability to measure rapid O2 and
lactate changes as surrogate marker of oxidative phosphorylation and anaerobic