The present study was focused on using HP 13C MRSI to detect noninvasively metabolic changes in diabetic liver rats fed with a high-fat diet. Planned treatment is 20 weeks to induce NASH, but we present interim data at 4 weeks of treatment. Liver fat signal fraction was significantly increased. In animals, in which the fat and body weight increased, lactate/pyruvate ratio was significantly decreased. This might be explained by a stimulation of gluconeogenesis by high levels of fatty acids in the liver. Long-term monitoring allows us a better understanding of the metabolic changes in the progression from NAFLD diabetic rats.