Abstract #4712

Glymphatic monitoring in awake state mouse brain with DCE-MRI imaging

Niklas Daniel Åke Persson¹, Evan Hunter Stanton ¹, Björn Sigurdsson¹, Tuomas Lilius¹, Humberto Mestre², Maiken Nedergaard^1,2, and Yuki Mori^1,3

¹Division of Glial Disease and Therapeutics, University of Copenhagen, Center for Translational Neuromedicine, Copenhagen, Denmark, ²Division of Glial Disease and Therapeutics, University of Rochester Medical Center, Center for Translational Neuromedicine, Rochester, NY, United States, ³Faculty of Health and Medical Sciences, University of Copenhagen, Panum NMR Core Facility, Copenhagen, Denmark

The glymphatic pathway is a novel pathway for interstitial solute clearance. Previous reports note that glymphatic patterns differ according to brain activity. Due to the motion-induced image blurring effects, DCE-MRI in the awake animal is difficult. We aimed to determine the feasibility of FISP-based fast DCE-MRI to assess the glymphatic pathway in awake mice. Head-fixed mice were habituated to restraint and received a cisterna magna cannulation. Awake DCE-MRI was performed 24 hours post surgery. Dynamic tracer distribution maps were calculated, and tracer distribution was measured in each brain segmentation. We successfully obtain DCE-MRI and demonstrate glymphatic dynamics in awake mice.

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