Characterizing the physiological environments of a prostate cancer xenograft in orthotopic and subcutaneous sites
Bhujwalla Z, Artemov D, Ballesteros P, Raman V, Pathak A, Solaiyappan M
The Johns Hopkins University School of Medicine
Invasive human cancer xenografts invade and metastasize more efficiently following orthotopic implantation compared to heterotopic subcutaneous implantation. We recently generated a stably transfected human prostate cancer cell line with green fluorescent protein (GFP) expression under the control of a hypoxia response element (HRE) and have characterized its response to hypoxia in vivo. Here we have used this cell line to characterize differences in the orthotopic and heterotopic environments of this xenograft model using MRSI (total choline, lactate/lipid, and extracellular pH), and optical detection of GFP expression (hypoxia).